Recovery of lethally irradiated dogs following infusion of autologous marrow stored at low temperature in dimethylsulphoxide.

By JOHN A. CAVINS, SHINPEI KASAKURA, E. DONNALL THOMAS AND JOSEPH W. FERREBEE D OGS lethally exposed to general radiation usually make uneventful recoveries if given prompt infusions of a few billion autologous marrow cells.1’2 Cells for these infusions can be preserved indefinitely at bow temperature in glycerol. ’4 Recent studies suggest that dimethyl-sulphoxide may be preferable to glycerol for these preservations. 8 This communication reports the survival of dogs given 1200 r and infusions of autologous marrow stored at -80 C. in 10 per cent dimethyl-sulphoxide.